pX330A-1x3

CRISPR Mouse - Deletion ATDC5 Perk

Experiment
CRISPR Mouse - Deletion ATDC5 Perk
Product
pX330A-1x3 from Addgene
Manufacturer
Addgene

Protocol tips

Upstream tips
To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip (20uL or 200uL one), puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate.

Publication protocol

Genome editing using the multiplex CRISPR/Cas9 assembly system
The specific gRNA sequences were selected using the CRISPR design tool (http://crispr.mit.edu/) and cloned into the pX330A-1 × 3 multiplex gRNA assembly vector (Addgene plasmid #58767)50 using the Golden Gate cloning method. Then, the multiplex gRNA cassette was replaced with the lentiviral vector LentiGuide-Puro (Addgene plasmid #52963). Stable expression of Cas9 and gRNAs was established via puromycin or blasticidin resistance using LentiCas9-Blast (Addgene plasmid #529632) following Zhang et al.’s protocol51.

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Reviews

pX330A-1x3 from Addgene has not yet been reviewed for this experiment

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Discussion

Discussion

4 years ago

Author: Mario Udinese Italy

Floxing mice with CRISPR

Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?

Discussion

5 years ago

Author: Ben Saar Israel

How to choose a region to target for CRISPR

Hi everyone! I am planning on floxing mice with CRISPR but I am having trouble deciding which region to target. Do you have any tips on choosing?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing CRISPR Mouse - Deletion ATDC5 Perk using pX330A-1x3 from Addgene.

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Manufacturer protocol

Download the product protocol from Addgene for pX330A-1x3 below.

Download PDF Download manufacturer protocol

Videos

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